The Fusability of Erythrocytes as a Method for Evaluating the Instability of Their Membranes
| Autor: | Methun M. Rogozin, Gregory Ya. Levin, Aleksandra N. Popovicheva, Yury A. Sheremet’ev |
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| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Open Journal of Biophysics. :198-200 |
| ISSN: | 2164-5396 2164-5388 |
| Popis: | A novel approach to the study of erythrocytes instability based on assessment fusability of their plasma membrane is presented. The fusability of erythrocytes normal blood, stored for 7 days at 4°C, and type 2 patients with diabetes were studied. Human erythrocytes were fused by incubation with La3+ at final concentrations from 50 to 200 μM. Erythrocytes aggregates were incubated at 37°C for 120 min. The fusability of erythrocytes was evaluated using a light microscope. It was shown that La3+ induced extensive fusion of erythrocytes after 7 days of blood storage and erythrocytes of patients with diabetes. Incubation of normal erythrocytes at 37°C for 120 min does not induce cell fusion. |
| Databáze: | OpenAIRE |
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