Assessment of the distribution of nucleic acid intercalators in yeast cells by pseudospectral image analysis

Autor:	M. McCarren, E. O. Puchkov
Rok vydání:	2011
Předmět:	Mitochondrial DNA Saccharomyces cerevisiae Biophysics Biology biology.organism_classification chemistry.chemical_compound chemistry Biochemistry Nucleic acid Fluorescence microscope DAPI Binding site Ethidium bromide DNA
Zdroj:	Biophysics. 56:651-655
ISSN:	1555-6654 0006-3509
DOI:	10.1134/s0006350911040233
Popis:	The intracellular location of nucleic acid intercalators (NAI) in native (not fixed) Saccharomyces cerevisiae cells has been studied using fluorescence microscopy combined with computer pseudospectral image analysis. Three NAI: anthracycline anticancer drug doxorubicin and nucleic acid dyes ethidium bromide and 4′,6-diamidino-2-phenylindole (DAPI) were used. All three NAI were shown to be localized in nuclei and mitochondria. In contrast to DAPI, which interacted only with DNA, a large fraction of doxorubicin and ethidium bromide apparently bound to mitochondrial membranes. Upon combined application, competition between these intercalators for binding sites in the nuclear and mitochondrial DNA occurred. It was concluded that this approach may be used in designing new DNA-targeted drugs and in preliminary studies of their interaction with eukaryotic cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9779502b9d6384385721a79d203fb4e2 https://doi.org/10.1134/s0006350911040233 Zobrazit plný text záznamu Full text from SpringerLink