Analysis of serum proteins electrophoresis in clinically healthy miniature Caspian horse

Autor: Nahid Atyabi, Ehsan Torki, Mohamad Naser Nazem, Mohamad Zamani Ahmadmahmudi
Rok vydání: 2011
Předmět:
Zdroj: Comparative Clinical Pathology. 21:795-799
ISSN: 1618-565X
1618-5641
Popis: The Iranian miniature Caspian horse is the most ancient domestic breed of horse; it is the likely ancestor of all modern breeds of hot-blooded horses. Blood serum protein analysis has not been sufficiently studied in miniature Caspian horses. Since serum electrophoresis may be an important preclinical tool for diagnosis of equine diseases, evaluation of serum protein fractions in miniature Caspian horses using acetate cellulose strips was undertaken. In addition, serum protein concentrations were also examined. Blood samples were collected from 30 clinically healthy horses with and without anticoagulant. Using cellulose acetate electrophoresis (CAE), serum samples were fractionated and absolute and relative concentrations from each sample calculated. From 30 samples, 26 samples (86.6%) had five protein fractions and four samples (13.4%) had four fractions. Samples with five fractions were a common pattern (χ 2, P 0.05). Absolute values of albumin, α1, α2, β, and γ globulin were 2.9 ± 0.24, 0.97 ± 0.19, 0.96 ± 0.24, 1.2 ± 0.36, and 1.3 ± 0.29 g/dl, respectively. Mean albumin to globulin ratio was 0.67. In conclusion, analysis of serum proteins in the miniature Caspian horse using CAE showed five protein fractions including albumin, α1, α2, β, and γ globulin.
Databáze: OpenAIRE
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