Autor:	Vaclav Zidek, V. Bila, Hein A. van Lith, Bert F. M. Van Zutphen, Vlastimil Kostka, Michal Pravenec, Anita Bonne, Alena Musilova, Drahomira Krenova, Maria den Bieman, Vladimir Kren, G. F. Gillissen
Rok vydání:	2003
Předmět:	Genetics General Medicine Biology Biochemistry Molecular biology law.invention Inbred strain Genetic linkage law Genetic marker Recombinant DNA Amplified fragment length polymorphism Primer (molecular biology) Molecular Biology Ecology Evolution Behavior and Systematics
Zdroj:	Biochemical Genetics. 41:77-89
ISSN:	0006-2928
DOI:	10.1023/a:1022069816135
Popis:	The amplified fragment length polymorphism (AFLP) technique has been used to enhance marker density in a large set of recombinant inbred strains (H x B and B x H) derived from a spontaneously hypertensive rat (SHR/OlaIpcv) and a Brown-Norway (BN.lx/Cub) inbred strain. Thirteen different primer combinations were tested and a total of 191 polymorphic bands were detected. From these polymorphic bands 89 AFLP markers could be assigned to specific chromosomes. Several of these AFLP markers were mapped to regions with low marker density, thus filling up gaps in the existing genetic map of these recombinant inbred strains. These results substantiate the value of the AFLP technology in increasing marker density in genetic maps.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::974dc31c36fe87f86d7d8388851799c2 https://doi.org/10.1023/a:1022069816135 Zobrazit plný text záznamu Full text from SpringerLink