| Autor: |
Yue-Xiu Dang, Yuliang Wang, Du Wenjia, Cheng-Jun Zhang, Shuan-hu Lei, Li Guo, Jing Wang, Jun-long Hao, Da-Cheng Zhao, Liu Jingsheng, Hai-yuan Yue, Liang-Zeng Huang |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Orthopaedic Surgery. 5:280-288 |
| ISSN: |
1757-7853 |
| DOI: |
10.1111/os.12071 |
| Popis: |
2013 Chinese Orthopaedic Association and Wiley Publishing Asia Pty Ltd.OBJECTIVE: To investigate the influence of bone marrow stromal stem cell (BMSCs) transplantation on healing of fractures combined with central nerve injuries in rats.METHODS: Forty-eight healthy adult SD male rats were randomly divided into the following three groups (16 rats in each group): group A, simple (left) tibial fracture; group B, tibial fracture combined with T10 spinal cord transection (SCT); group C, tibial fracture combined with T10 SCT and BMSCs transplantation. The tibial fractures were stabilized with modular intramedullary nails and all operated hind limbs were further immobilized in plaster casts to prevent unequal load bearing. BMSCs were labeled with bromodeoxyuridine and implanted into the fractures of C group rats 2 days after creation of the model. The animals in B and C groups were evaluated by postoperative Tarlov scores. The fractured tibiae were evaluated separately radiographically (X-ray and CT) and immunohistochemically 1, 2, 3 and 4 weeks after injury to assess fracture healing. In addition, the wet weights of the left tibias were measured.RESULTS: All Tarlov score of the B and C group animals reached the requirements of the experiment. One, 2 and 3 weeks after surgery, the tibial callus widths in B and C group animals were significantly greater than those of group A rats (P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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