A Study of the Interaction Between a New Reagent and Serum Albumin by Fluorescence Spectroscopy

Autor:	Fengling Cui, Donglan Ma, Jing Fan, Mancang Liu, Zhide Hu, Xingguo Chen
Rok vydání:	2003
Předmět:	Chromatography biology Biochemistry (medical) Clinical Biochemistry Fluorescence spectrometry Albumin Serum albumin Human serum albumin Biochemistry Fluorescence spectroscopy Analytical Chemistry chemistry.chemical_compound Thiourea chemistry Reagent Electrochemistry medicine biology.protein Bovine serum albumin Spectroscopy medicine.drug
Zdroj:	Analytical Letters. 36:2151-2166
ISSN:	1532-236X 0003-2719
DOI:	10.1081/al-120023708
Popis:	The synthesis of a new reagent, saturated fatty hydrocarbon substituting group compound N-n-hexyl-N ′-(sodium p-aminobenzenesulfonate) thiourea (HXPT), is described. The interactions between HXPT and bovine serum albumin or human serum albumin were studied by fluorescence spectroscopy. The binding constants of HXPT with BSA or HSA were determined at different temperatures under the optimum conditions. The binding sites were obtained and the acting force were suggested to be mainly hydrophobic. The effect of common ions on the binding constants was also investigated. A practical method was proposed for the determination of HXPT in bovine serum or human serum samples.
Databáze:	OpenAIRE
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