O-040 Investigating why early embryonic mitoses are highly error prone by real time imaging
| Autor: | J Ellenberg |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Human Reproduction. 37 |
| ISSN: | 1460-2350 0268-1161 |
| Popis: | Maintenance of genomic integrity during early embryogenesis is essential for faithful embryonic development. However, mitotic errors during preimplantation development are surprisingly common and despite the fundamental importance of the cell divisions at the beginning of life, the mechanisms that control the correct progression through mitosis during pre-implantation development are not well characterized. The sensitivity of mammalian embryos to light and its demanding in vitro culture requirements have limited real-time imaging during the first embryonic divisions in the past. Today, advances in light-sheet microscopy allow in toto imaging of preimplantation mouse development from zygote to blastocyst at single chromosome resolution and enable us to study spindle assembly, chromosome restructuring and segregation. This presentation will discuss the insights we have gained by real-time imaging of the first cell divisions in mammalian life, and the role of the key molecular mechanisms that regulate mitosis during the critical first stages of embryo development. |
| Databáze: | OpenAIRE |
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