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This study aimed to validate the use of two SNP markers associated to a sdw1 allele identified previously in a short barley genotype (ND23049) with an adequate peduncle extension which reduces predisposition to fungal disease development. First, the GBS SNP were converted in a KASP marker but only one of them, named TP4712, correctly amplified all allelic variations and had a mendelian segregation in a F2 population. To corroborate the association between TP4712 allele with plant height and peduncle extension a total of 842 genotypes were genotyped and evaluated for both traits. Out of the 842 genotypes, 199 were F4 lines, 79 were a diverse panel and 564 were a complete breeding cohort of stage 1 yield trials. To corroborate the association between the sdw1 allele and the short plant height with adequate peduncle extension, contingency tables were created, grouping the 1954 data points into categories. Based on the contingency analysis it was demonstrated that the higher proportion of short plants with adequate peduncle extension were present in genotypes carrying the SNP allele present in ND23049 regardless the population and the sowing date. This study develops a marker assisted selection tool to accelerate the introgression of favourable alleles for plant height and peduncle extension in adapted germplasm. |
