Evidence in support of a virus etiology for bovine leukemia

Autor: Clarita P. Eusebio, Robert R. Marshak, Keyvan Nazerian, Ray M. Dutcher, Gerald B. Guest, Joseph J. Tumilowicz, Edward P. Larkin, Neale D. Stock
Rok vydání: 1967
Předmět:
Zdroj: Cancer. 20:851-856
ISSN: 1097-0142
0008-543X
DOI: 10.1002/1097-0142(1967)20:5<851::aid-cncr2820200544>3.0.co;2-o
Popis: Virus-like particles resembling the C-type particles of murine leukemia were detected in tissue biopsies and more frequently in thin sections of pellets following density gradient centrifugation of milk from leukemic cows and cows in a high incidence leukemic herd. These particles had a buoyant density in potassium citrate and sucrose gradients of 1.15 to 1.16 Gm/cc. Fractions in which the particles were found contained both DNA and RNA, with RNA in higher concentration. Virus interference assays on tissue cultures derived from leukemic and leukemia-free cattle yielded equivocal results. Bone marrow cultures were uniformly resistant to challenge with vesicular stomatitis virus. Interferon was identified in the supernatant fluid of two resistant cultures. Immunofluorescence studies with anti-Rauscher virus globulin and a globulin from a leukemic calf suggested possible antigenic similarity when the fluorescent globulins were used to stain tissue cultures derived from leukemic cattle.
Databáze: OpenAIRE
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