New potentiometric sensors for creatinine
| Autor: | Mohamed A. F. Elmosallamy |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Detection limit
Creatinine Chromatography Chemistry Potassium Potentiometric titration Cationic polymerization chemistry.chemical_element Biochemistry Analytical Chemistry Matrix (chemical analysis) Solvent chemistry.chemical_compound Environmental Chemistry Quantitative analysis (chemistry) Spectroscopy |
| Zdroj: | Analytica Chimica Acta. 564:253-257 |
| ISSN: | 0003-2670 |
| Popis: | Three main types of creatinine potentiometric membrane sensors are described. They are based on the use of dibenzo-30-crown-10 (DB30C10) with potassium tetrakis( p -chlorophenyl)borate type (I), dibenzo-30-crown-10 alone type (II), and potassium tetrakis( p -chlorophenyl)borate alone type (III), incorporating in poly(vinyl chloride) matrix membrane plasticized with either o -nitrophenyl octyl ether or dioctylphthalate. The sensors are used for quantification of creatinine after soaking the membranes in 0.1 M creatinine solution for 2 days. The sensors show almost the same potentiometric response characteristics. Sensor type (I) exhibits Nernstian responses over a concentration range of 5.0 × 10 −5 mol l −1 –1.0 × 10 −2 mol l −1 creatinine with cationic slopes of 59.5 ± 0.1 and 60 ± 0.2 mV decade −1 and detection limits of 1.1 × 10 −5 mol l −1 and 8 × 10 −6 mol l −1 creatinine, over the pH range of 3.5–6.5 and 3.5–7.0, for o -NPOE and DOP solvent mediators, respectively. Sensor type (II) displays Nernstian responses over a concentration range of 6.0 × 10 −5 mol l −1 –1.0 × 10 −2 mol l −1 creatinine with cationic slopes of 60.0 ± 0.1 and 65.0 ± 0.2 mV decade −1 and detection limits of 1.5 × 10 −5 mol l −1 and 1.4 × 10 −5 mol l −1 creatinine over the pH range of 2.6–6.2 and 2.5–6.0, for o -NPOE and DOP solvent mediators, respectively. Sensor type (III) shows Nernstian responses over a concentration range of 7.0 × 10 −5 mol l −1 –1.0 × 10 −2 mol l −1 creatinine with cationic slopes of 60 ± 0.1 and 62.0 ± 0.2 mV decade −1 and detection limits of 2.7 × 10 −5 mol l −1 and 2.0 × 10 −5 mol l −1 creatinine over the pH range of 2.5–6.0, for o -NPOE and DOP solvent mediators, respectively. The response times of the sensors for 10 −3 mol l −1 creatinine solution are instantaneous (4–10 s). The sensors show long-term stability with life span of ∼6 months. The sensors are used for determination of serum creatinine of rats ( Rattus Norvigicus ) with mean R.S.D. of 2.62%, and the results agreed well with the Jaffe kinetic method. |
| Databáze: | OpenAIRE |
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