Vaterite bio-precipitation induced by Bacillus pumilus isolated from a solutional cave in Paiania, Athens, Greece

Autor: A. Magoulas, Aristomenis P. Karageorgis, Ioannis Katsikis, G. Kotoulas, Markos I. Daskalakis, Asterios Bakolas, A. Mavridou, Fotis Rigas
Rok vydání: 2015
Předmět:
Zdroj: International Biodeterioration & Biodegradation. 99:73-84
ISSN: 0964-8305
DOI: 10.1016/j.ibiod.2014.12.005
Popis: In this work, Bacillus pumilus ACA-DC 4061 was selected for its increased capability for biomineralization on marble, under different nutrient media concentrations and temperature conditions. The optimum conditions for the CaCO3 bacterially-induced precipitation were determined with the aid of testing based on the Design of Experiments (DoE). Biomineral (vaterite) precipitation was favored in both the temperatures (25 and 30 °C) investigated. Stone loss rate was reduced when the samples were subjected to sonication. Thin sections of the substrate confirmed that vaterite was able to adhere onto the surface. Finally, under non-sterile conditions mimicking an in situ application, B. pumilus ACA-DC 4061 formed a fine layer of calcium carbonate. Therefore, this microorganism showed that vaterite formation may consistently occur under specific conditions and could prove useful as a candidate for on-site applications for stone conservation.
Databáze: OpenAIRE