Pseudomonas C12B, an SDS degrading strain, harbours a plasmid coding for degradation of medium chain length n-alkanes

Autor: Dani L. McBeth, Miloslav Suchanek, Hana Klierova, Jan Kostal, Katerina Demnerova, Blanka Kralova
Rok vydání: 1998
Předmět:
Zdroj: International Biodeterioration & Biodegradation. 42:221-228
ISSN: 0964-8305
DOI: 10.1016/s0964-8305(98)00053-5
Popis: Pseudomonas C12B is able to degrade alkyl sulfates, alkylbenzene sulfonates, and linear alkanes and alkenes. Mitomycin C curing experiments and conjugation experiments demonstrated that the ability to utilize n -alkanes (C 9 –C 12 ) and n -alkenes (C 10 and C 12 ) of medium chain length was plasmid-encoded. The plasmid was designated pDEC. Its size was estimated at several hundreds kb according to mobility in agarose gels. The plasmid did not confer resistance to the antibiotics tested. Analysis of alkylsulfatases P1 and P2 in original and cured strains confirmed that both enzymes are encoded by the chromosome. The ability of Pseudomonas C12B to utilize alkylbenzene sulfonates also appears to be encoded by the chromosome. pDEC could be transferred only to cured derivatives of Pseudomonas C12B, but not to strains of P. aeruginosa , P. putida, or Acinetobacter sp. Cured derivatives of Pseudomonas C12B could not serve as hosts for the broad host range plasmid CAM–OCT. The enzyme system encoded by the putative dec genes present on plasmid pDEC differs from the system coded by the alk genes of plasmid OCT in the size range of hydrocarbons preferentially used.
Databáze: OpenAIRE
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