In vitro morphogenesis and micropropagation of Aechmea ramosa var. ramosa Mart. ex Schult. f. (Bromeliaceae) from leaf explants

Autor:	Elisa Mitsuko Aoyama, Elias Terra Werner, Taís Cristina Bastos Soares, Andreia Barcelos Passo Lima-Gontijo, Rosiane Cipriano, Daniele Vidal Faria, Mariela J. Simão
Rok vydání:	2018
Předmět:	0106 biological sciences biology Plant tissue culture fungi food and beverages Bromeliaceae 04 agricultural and veterinary sciences Plant Science biology.organism_classification 01 natural sciences Murashige and Skoog medium Micropropagation Shoot Ornamental plant Botany 040103 agronomy & agriculture 0401 agriculture forestry and fisheries Aechmea ramosa 010606 plant biology & botany Biotechnology Explant culture
Zdroj:	In Vitro Cellular & Developmental Biology - Plant. 54:530-536
ISSN:	1475-2689 1054-5476
Popis:	Aechmea ramosa Mart. ex Schult. f. is an endemic bromeliad of the Brazilian Atlantic Forest. The current habitat degradation of this hotspot biome threatens this species, which besides having an important ecological role, is also of invaluable ornamental interest. Plant tissue culture has been used in mass propagation and conservation of various bromeliads. We have established a micropropagation protocol for A. ramosa var. ramosa using leaf explants grown in MS medium supplemented with 2 μM of 1-naphthaleneacetic acid (NAA) and 2 μM of 6-benzylaminopurine (BAP) that showed higher values of shoot induction. NAA and BAP are associated with the production of proteins involved in stress response modulation, metabolic activity, and cell division, the latter being involved in inducing the differentiation of competent cells. After 120 d of culture, each explant presented 28.9 shoots with an average size of 27.8 mm, with no variation in either Stomatal Index or density of the regenerated shoots. Plantlets measuring above 15-mm height were successfully acclimatized, presenting 100% survival rate. Thus, this protocol can be used for mass propagation of A. ramosa, and to supply demand for the market of ornamental plants. Furthermore, it represents an important tool for the conservation of this species and maintenance of an in vitro germplasm.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::96de026442ae1e321b134f83367f2232 https://doi.org/10.1007/s11627-018-9907-0 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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