Preparation of protoplasts from the cyanobacterium Spirulina platensis and a novel viability assay
| Autor: | T.N. Prabha, K.M. Priya Sethu, L.V. Venkataraman |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
Spirulina (genus) Protoplast Biology biology.organism_classification Applied Microbiology and Biotechnology Superoxide dismutase chemistry.chemical_compound Enzyme Biochemistry chemistry Phycocyanin medicine biology.protein Viability assay Mannitol Lysozyme medicine.drug |
| Zdroj: | Letters in Applied Microbiology. 18:241-244 |
| ISSN: | 1472-765X 0266-8254 |
| DOI: | 10.1111/j.1472-765x.1994.tb00859.x |
| Popis: | [Cyanobacterial genus Spirulina produces proteins, beta-carotene, vitamins (including B12), and other commercially important products, e.g. phycocyanin, gamma-linolenic acid, and superoxide dismutase. For commercial purposes, improvement of strains through genetic manipulation is desirable.] A method is described for the isolation of protoplasts from Spirulina platensis using an elite strain (FT 06) selected after screening. Trichomes of the cyanobacterium were treated with lysozyme (0.1%) for 28 h at room temp. in 0.03 mol/l phosphate buffer (pH 6.8) with 0.5 mol/l mannitol as the osmoticum. This resulted in an 80% yield of protoplasts. Cells were intact in their fine structure and viable as evidenced by a rapid and efficient viability assay using a novel mixture of fluorescein diacetate and calcofluor white. |
| Databáze: | OpenAIRE |
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