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Skin cancer is the most common type of cancer caused by uncontrolled growth of abnormal cells in the epidermis and the outermost skin layer. This work aimed at determining the anticancer activity of [6]-Gingerol, a bioactive compound from rhizome of Zingiber officinale and its structural analogs against skin cancer. The ethanolic crude extract of the selected plant was subjected to phytochemical and GC-MS analysis to confirm the presence of the [6]-Gingerol. The anticancer activity of extract was evaluated by MTT (3-[4, 5-dimethylthiazol-2-y]-2, 5-diphenyl tetrazolium bromide) assay using the A431 Human skin Adenocarcinoma cell line. The GC-MS analysis confirmed the presence of [6]-Gingerol compound and its promising cytotoxicity IC50 was found at 81.46 ug/ml in the MTT assay. Further, the in silico studies used [6]-Gingerol and 21 structural analogs collected from PUBCHEM database to investigate the anticancer potential and drug likeliness property. Skin cancer protein, DDX3X was selected as target which regulates all stages of RNA metabolism. It was docked with 22 compounds which includes [6]-Gingerol and 21 structural analogs, the potent lead molecule was selected based on lowest binding energy value. Thus the [6]-Gingerol and its structure analogs could be used as lead molecule against skin cancer and future drug development process. |
