Direct Observation of Postadsorption Aggregation of Antifreeze Glycoproteins on Silicates

Autor:	A. L. DeVries, J. J. Ramsden, ‡ C.-C. C. Cheng, and S. Scheuring, Ph. Lavalle
Rok vydání:	2000
Předmět:	Aqueous solution Chemistry Muscovite Surfaces and Interfaces engineering.material Condensed Matter Physics Amorphous solid Crystallography Adsorption Silicate minerals Desorption Electrochemistry engineering Molecule General Materials Science Mica Spectroscopy
Zdroj:	Langmuir. 16:5785-5789
ISSN:	1520-5827 0743-7463
Popis:	The adsorption of antifreeze glycoproteins (AFGP) from aqueous solution onto two different silicate minerals, muscovite mica and amorphous silica−titania, has been directly observed for the first time in situ using atomic force microscopy (AFM) and optical waveguide lightmode spectroscopy (OWLS). The former yields the lateral distribution and heights of adsorbed single molecules and massed adsorpta, and the latter enables the precise number of adsorbed AFGP per unit area of surface to be determined as well as the kinetics of adsorption and desorption. On both surfaces the AFGP were initially deposited as isolated molecules. On mica they remained as such and could be imaged as compact, globular objects, in contrast to the elongated form presumed to exist in solution. On silica−titania they subsequently formed isolated conical deposits containing large numbers of molecules. The relevance of these results to the adsorption of the AFGP on ice is discussed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::96d4c9586f1bf449f72692c6345ac931 https://doi.org/10.1021/la9916934 Zobrazit plný text záznamu