Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors

Autor:	Inbar Friedrich Ben-Nun, Marites T. Woon, Puspa R. Pandey
Rok vydání:	2021
Předmět:	Cell therapy Chemistry technology industry and agriculture Bioreactor Microcarrier equipment and supplies Induced pluripotent stem cell complex mixtures Cell biology
Zdroj:	Methods in Molecular Biology ISBN: 9781071620175
Popis:	Bioreactor technolology enables the expansion of mammalian cells, which can be translated to processes compatible with Current Good Manufacturing Practice (cGMP) regulations. Cells are introduced to the bioreactor vessel, wherein key parameters such as temperature, pH, and oxygen levels are tightly controlled to facilitate growth over time. Here, we describe the microcarrier-based expansion of human pluripotent stem cells in a 3 L stirred tank bioreactor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::96c31f2ea40c2c715c7c16bebd7b1160 https://doi.org/10.1007/7651_2021_396 Zobrazit plný text záznamu