| Autor: |
Guan, Fiona, Bailey, Charles, Metierre, Cynthia, O’Young, Patrick, Dadi Gao, Teh Khoo, Holst, Jeff, Rasko, John |
| Rok vydání: |
2017 |
| Předmět: |
|
| DOI: |
10.6084/m9.figshare.c.3728662_d7 |
| Popis: |
Confirmation of DNA binding of ELF2 isoforms by ChIP. A) The doxycycline-regulatable ‘dox-off’ lentiviral vector used to co-express eGFP and ELF2 isoforms. B) Flow cytometric analysis of HEK293T cells transfected with eGFP only (control)-, HA-ELF2A- and HA-ELF2B-containing vectors. C) ChIP PCR of known ELF2 targets (VCP, PYGO2, LMO2, and LYN promoters), novel ELF2-binding sites in ELF2 promoter regions (P1, P2, and P3) as well as a negative control region spanning H19 exons 4 and 5. (PDF 1124 kb) |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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