Embryogenic Callus Formation from Protoplasts Derived from Suspension Cells of Apomictic Guineagrass (Panicum maximum Jacq.)

Autor:	Franz Hoffmann, Siegfried Lachmann, Taiji Adachi, Ryo Akashi
Rok vydání:	1995
Předmět:	Sucrose Somatic embryogenesis Cell division Callus formation fungi food and beverages Biology Protoplast chemistry.chemical_compound Murashige and Skoog medium chemistry Callus Botany Leafy
Zdroj:	Japanese Journal of Breeding. 45:445-448
ISSN:	2185-291X 0536-3683
Popis:	Protoplasts were isolated from suspension cells of apouuictic guineagrass (Pauicum maximum Jacq.). The suspension culture used as donor Inaterial was originally initiated from immature embryoderived embryogenic callus. Prior to protoplast isolation, suspension cells were conditioned with Murashige and Skoog (MS) Iiquid medium without sucrose and growth regulators. This pretreatment lead to a dramatic increase in protoplast yield and colony formation. Cell division and colony formation from such pretreated protoplasts were found to be best in agarose-solidified modified KM8p medium. Protoplast derived colonies developed into callus on solidified MS medium supplemented with 1 mg/l 2, 4-D. After 2 months in culture, calli formed compact white nodular structures some of which developed into somatic embryos. Although some of the somatic embryos developed small leafy structures, whole plants could not be regenerated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::96a098d3cceeb92943ded8800bffeb3c https://doi.org/10.1270/jsbbs1951.45.445 Zobrazit plný text záznamu