Acute effects of acidosis on protein and amino acid metabolism in perfused rat liver
| Autor: | Jana Kadlcikova, Radana Rysava, Roman Safranek, Milan Holecek, L. Šprongl |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Protein metabolism Metabolic acidosis Cell Biology Metabolism Biology medicine.disease Pathology and Forensic Medicine Amino acid chemistry.chemical_compound Endocrinology chemistry Biochemistry Valine Internal medicine Ketogenesis medicine Leucine medicine.symptom Molecular Biology Acidosis |
| Zdroj: | International Journal of Experimental Pathology. 84:185-190 |
| ISSN: | 0959-9673 |
| DOI: | 10.1046/j.1365-2613.2003.00352.x |
| Popis: | Acidosis is frequently associated with protein wasting and derangements in amino acid metabolism. As its effect on protein metabolism is significantly modulated by other abnormal metabolic conditions caused by specific illnesses, it is difficult to separate out the effects on protein metabolism solely due to acidosis. The aim of the present study was to evaluate, using a model of isolated perfused rat liver, the direct response of hepatic tissue to acidosis. We have compared hepatic response to perfusion with a solution of pH 7.2 and 7.4 (controls). Parameters of protein and amino acid metabolism were measured using both recirculation and single-pass technique with 4,5-[3H]leucine, [1-14C]leucine and [1-14C]ketoisocaproate (ketoleucine) as tracers and on the basis of difference of amino acid levels in perfusion solution at the beginning and end of perfusion. In liver perfused with a solution of pH 7.2, we observed higher rates of proteolysis, protein synthesis, amino acid utilization and urea production. Furthermore, the liver perfused with a solution of pH 7.2 released a higher amount of proteins to perfusate than the liver perfused with a solution of pH 7.4. Enhanced decarboxylation of ketoisocaproate in liver perfused by a solution of a lower pH indicates increased catabolism of branched-chain amino acids (leucine, valine and isoleucine), decreased reamination of branched-chain keto acids to corresponding essential amino acids and increased ketogenesis from leucine. |
| Databáze: | OpenAIRE |
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