A Novel Disposable Biosensor Based on SiNWs/AuNPs Modified-Screen Printed Electrode for Dengue Virus DNA Oligomer Detection

Autor:	Uda Hashim, Jaafar Abdullah, Reza Hajian, Nor Azah Yusof, Jahwarhar Izuan Abd Rashid
Rok vydání:	2015
Předmět:	Detection limit Materials science Analytical chemistry Oligomer Redox indicator chemistry.chemical_compound chemistry Colloidal gold Electrode Differential pulse voltammetry Electrical and Electronic Engineering Cyclic voltammetry Instrumentation Biosensor Nuclear chemistry
Zdroj:	IEEE Sensors Journal. 15:4420-4427
ISSN:	1558-1748 1530-437X
DOI:	10.1109/jsen.2015.2417911
Popis:	In this paper, a disposable screen-printed gold electrode (SPGE) utilized of silicon nanowires (SiNWs) and gold nanoparticles as sensing material was fabricated for detection of DNA oligomers related to dengue virus. First, SiNWs/AuNPs-SPGE was developed by the dispersion of SiNWs in 3-aminopropyltriethoxysilane (0.5%) onto bare SPGE. Second, the AuNPs decoration on SiNWs-SPGE surface was functionalized using dithiopropionic acid through a self-assembly monolayer technique. The electrochemical response of methylene blue (MB) as a redox indicator toward synthetic DNA oligomer after hybridization on SiNWs/AuNPs-SPGE was recorded by cyclic voltammetry and differential pulse voltammetry techniques. The results demonstrated that the reduction peak current of MB was significantly decreased after DNA hybridization process. In addition, the developed biosensor showed a good storage stability and could achieve a linear range of $1 \times 10^{-11}-1 \times 10^{-7}$ M ( $R= 0.98$ ) with the detection limit of $1.63 \times 10^{-12}$ M.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::96971602f9cba0a93bef5161fe5a371b https://doi.org/10.1109/jsen.2015.2417911 Zobrazit plný text záznamu