Nasal Immunization with P. gingivalis Surface Protein Antigen and Cholera Toxin Adjuvant Induces T Helper 2 Responses in Both Mucosal and Systemic Compartments
| Autor: | Mitsuo Hayakawa, Yoshimitsu Abiko, Masafumi Yamamoto, Satomi Maeba, Shigeo Otake, Jun Namikoshi |
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| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_treatment
Cholera toxin chemical and pharmacologic phenomena Spleen Biology bacterial infections and mycoses medicine.disease_cause biology.organism_classification complex mixtures In vitro Cytokine medicine.anatomical_structure Antigen Immunology medicine bacteria Nasal administration Adjuvant Porphyromonas gingivalis |
| Zdroj: | International Journal of Oral-Medical Sciences. 1:90-96 |
| ISSN: | 2185-4254 1347-9733 |
| DOI: | 10.5466/ijoms.1.90 |
| Popis: | It is well established that Porphyromonas gingivalis is one of the major pathogens of adult periodontitis. P. gingivalis produces an outer membrane protein with a molecular mass of 40-kDa (40k-OMP). In the present study, in order to assess the potential for application of 40k-OMP in the development of an anti-periodontal disease vaccine, we analyzed 40k-OMP-specific CD4+ T helper (Th) cell responses induced in the mucosal as well as systemic compartments when 40k-OMP was administered nasally. When CD4+ T cells that were isolated from cervical lymph nodes (CLN) and spleens of mice immunized with 40k-OMP plus cholera toxin (CT) as adjuvant were re-stimulated with 40k-OMP in vitro, significant levels of proliferative responses were induced. In contrast, essentially no increased proliferation occurred in CLN and spleens taken from mice given 40k-OMP alone. Analysis of T helper (Th) 1 (IFN-γ) and Th2 (IL-4, IL-5 and IL-6) cytokine responses showed that 40k-OMP-specific Th cells from both CLN and the spleen produced significant levels of IL-4, IL-5 and IL-6 but did not result in changes in IFN-γ production. In contrast, marginal levels of IL-4, IL-5 and IL-6 production were seen in mice given 40k-OMP alone nasally. These results suggest that nasal administration of 40k-OMP plus CT as an adjuvant can elicit 40k-OMP-specific Th2-type cytokine responses in both mucosal and systemic compartments. Further, the nasal 40k-OMP vaccine possesses the potential for antiperiodontal vaccine. |
| Databáze: | OpenAIRE |
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