| Autor: |
Basavaraj S. Nainegali, Badekar Sagar Dilip, Bommenahalli Shashidhara Rashmi, Iyyaswami Regupathi |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Biotechnology and Biochemical Engineering ISBN: 9789811019197 |
| DOI: |
10.1007/978-981-10-1920-3_11 |
| Popis: |
Aqueous two-phase extraction (ATPE) is unique type of liquid-liquid extraction process used for the selective partitioning of high value biological products at a highly purified form with low cost and energy consumption. An Acetonitrile-potassium citrate aqueous two-phase system was studied because of its low value, simplification of product recovery from the top acetonitrile-rich phase and possibility of recycling the phase materials. The binodal and equilibrium data were generated at 303.15 K for the construction of phase diagram. Further the experimental binodal data were analyzed and used to develop the relevant correlations. The liquid–liquid equilibrium data were predicted through the Othmer–Tobias correlations. The partition ability of the Acetonitrile—potassium citrate system was accessed through the partition behaviour of Bovine Serum Albumin (BSA). The effects of acetonitrile and salt concentrations, tie line length (TLL), pH and phase volume ratio were studied on the partitioning coefficient and recovery of BSA. The experimental data revealed that the Acetonitrile—potassium citrate ATPs able to partition the BSA in acetonitrile rich phase in a one step with partition coefficient higher than 7.0 and nearly about 89 % recovery. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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