| Autor: |
Katendi Changula, Walter Muleya, Karen Sichibalo, Natasha Katuta Mwila, Simbarashe Chitanga, King S. Nalubamba, Kennedy Chibesa, Benjamin Mubemba, Chisoni Mumba, Edgar Simulundu |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.21203/rs.3.rs-1082371/v1 |
| Popis: |
Acaricide resistance in ticks poses a great threat to livestock production in many parts of the world where ticks are a problem. The objectives of this study were to screen for acaricide resistance in Rhipicephalus and Amblyomma ticks using phenotypic and molecular assays, and to assess current tick control practices used by cattle farmers in the traditional sector of Namwala District. The larval packet test was used to screen for acaricide resistance in ticks covering concentrations up to twice the discriminatory dose for amitraz, diazinon and cypermethrin. Genetic mutations associated with resistance to amitraz (A22C-T8P and T65C-L22S), and organophosphates/ pyrethroids (G1120A) were screened using polymerase chain reaction and sequencing. Information on tick control practices at the household level was collected using a semi-structured questionnaire. Intermediate resistance (21-50%) to amitraz and cypermethrin was detected in both Rhipicephalus and Amblyomma ticks, with both tick genera showing susceptibility to diazinon ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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