The quantitative analysis of seed proteins from peas using high performance liquid chromatography
| Autor: | James R. Bacon, Stephen J. Chambers, Nigel Lambert |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Gel electrophoresis
Chromatography Globulin biology Chemistry Elution Albumin food and beverages Plant Science General Medicine Biochemistry High-performance liquid chromatography Analytical Chemistry Complementary and alternative medicine Drug Discovery Vicilin biology.protein Molecular Medicine Legumin Quantitative analysis (chemistry) Food Science |
| Zdroj: | Phytochemical Analysis. 3:49-54 |
| ISSN: | 1099-1565 0958-0344 |
| DOI: | 10.1002/pca.2800030202 |
| Popis: | Hydroxyapatite chromatography is frequently employed in the purification of pea seed proteins. By scaling down the procedure and interfacing it to a high performance liquid chromatographic (HPLC) system a method has been developed for the quantitative analysis of seed proteins not only from pea but potentially many other seeds. Under appropriate conditions three peaks were eluted from the column after first washing with a saline buffer. The peaks were shown by SDS-polyacryamide gel electrophoresis to correspond to albumin, legumin and vicilin respectively. The technique provides results of a high degree of accuracy. Protein compositions were determined for six commercial pea varieties, with each analysis taking one hour and requiring less than one milligram of protein extract. Multiple loadings of the same sample indicated a very high level of reproducibility. The HPLC method revealed significantly higher levels of albumin compared with published values, and demonstrated the unreliability of fractionating globulins and albumins on the basis of solubility. The merits of this technique relative to alternative methods are discussed. |
| Databáze: | OpenAIRE |
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