Autor:	You-Qiang Wu, Alicja Ziemienowicz, Barbara Hohn
Rok vydání:	2001
Předmět:	chemistry.chemical_classification DNA ligase Plant Science General Medicine Biology Molecular biology Sequencing by ligation law.invention Enzyme chemistry Sticky and blunt ends Biochemistry law Genetics Recombinant DNA DNA supercoil Ligase chain reaction Agronomy and Crop Science Adenylylation
Zdroj:	Plant Molecular Biology. 46:161-170
ISSN:	0167-4412
DOI:	10.1023/a:1010679901911
Popis:	Here we report the purification and biochemical characterization of recombinant Arabidopsis thaliana DNA ligase I. We show that this ligase requires ATP as a source for adenylation. The calculated K m [ATP] for ligation is 3 μM. This enzyme is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates, but not in oligo(dT)/poly(rA) substrates. Double-stranded DNAs with cohesive or blunt ends are also good substrates for the ligase. These biochemical features of the purified enzyme show the characteristics typical of a type I DNA ligase. Furthermore, this DNA ligase is able to perform the reverse reaction (relaxation of supercoiled DNA) in an AMP-dependent and PPi-stimulated manner.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9658c8b008c1bf25a55431451d07d647 https://doi.org/10.1023/a:1010679901911 Zobrazit plný text záznamu Full text from SpringerLink