Chronic expression of P-selectin on endothelial cells stimulated by the T-cell cytokine, interleukin-3
| Autor: | Sarah Newlands, Michael C. Berndt, Yeesim Khew-Goodall, Eija Korpelainen, Mathew A. Vadas, Marek S. Litwin, Leanne Noack, Jennifer R. Gamble, Angel F. Lopez, Carolyn M. Butcher |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
P-selectin Endothelium medicine.medical_treatment T cell Immunology Cell Biology Hematology Biology Biochemistry Molecular biology Endothelial stem cell Cytokine medicine.anatomical_structure Thrombin Endocrinology Internal medicine medicine Tumor necrosis factor alpha Interleukin 3 medicine.drug |
| Zdroj: | Blood. 87:1432-1438 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v87.4.1432.bloodjournal8741432 |
| Popis: | P-selectin expressed on the surface of endothelium mediates leukocyte adhesion in vitro and rolling in vivo. Several inducers of cell-surface P-selectin expression on endothelial cells (EC) have previously been identified, all of which yield transient cell-surface expression of P- selectin lasting minutes to a few hours. We now show that a T- lymphocyte product, interleukin-3 (IL-3), stimulates the long-term endothelial cells (HUVEC). IL-3 induced cell-surface P-selectin expression in two phases. An initial peak at 10 minutes was followed by a prolonged upregulation beginning 16 hours after IL-3 addition and lasting at least 4 days. The level of P-selectin expression induced by IL-3 added for 48 hours was similar to that induced by treatment of HUVEC for 10 minutes with thrombin, and the effect of adding IL-3 for 48 hours followed by thrombin for 10 minutes was additive. Induction of cell-surface P-selectin expression by IL-3 was blocked by pretreatment of EC with a blocking monoclonal antibody against the IL-3 receptor alpha-chain. Lipopolysaccharide (LPS), tumor necrosis factor alpha (TNF alpha) and a mutant form of IL-3 with decreased potency did not induce cell-surface P-selectin expression after 48 hours' incubation with HUVEC, suggesting that the effect was specific to IL-3. The increase in cell-surface P-selectin expression occurring after 16 hours of incubation with IL-3 was accompanied by a similar prolonged increase in the steady-state mRNA level that was not observed at 10 minutes after IL-3 addition. As T-lymphocyte infiltration is a hallmark of chronic inflammation, our observations suggest that the secretion of IL-3 by T lymphocytes may serve to maintain the inflammatory state during chronic inflammation. |
| Databáze: | OpenAIRE |
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