Genome sequence of Acremonium strictum AAJ6 strain isolated from the Cerrado biome in Brazil and CAZymes expression in thermotolerant industrial yeast for ethanol production
| Autor: | Rosana Goldbeck, Gleidson Silva Teixeira, Francisco Maugeri Filho, Núria Adelantado, Alberto Moura Mendes Lopes, Allan Henrique Felix de Melo, Lucas Miguel de Carvalho, Marcelo Falsarella Carazzolle, Pau Ferrer, Gonçalo Amarante Guimarães Pereira, Dielle Pierroti Procópio |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences CAZy Acremonium strictum Bioengineering Cellobiose Biology biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology Biochemistry Yeast 03 medical and health sciences chemistry.chemical_compound chemistry Cellulosic ethanol 010608 biotechnology Ethanol fuel Glycoside hydrolase Food science Bagasse 030304 developmental biology |
| Zdroj: | Process Biochemistry. 98:139-150 |
| ISSN: | 1359-5113 |
| DOI: | 10.1016/j.procbio.2020.07.029 |
| Popis: | Increased demand for biofuels promotes the search for new biomass-degrading fungi. Acremonium strictum is an environmentally widespread filamentous fungi found on plant debris; that secretes lignocellulose-degrading enzymes. A recently isolated A. strictum strain, AAJ6; native to the Brazilian Cerrado biome was evaluated for its capacity to degrade lignocellulosic substrates. In this study, whole-genome sequencing of AAJ6 was performed and 775 CAZy domains were identified which correlated to those of A. strictum strain DS1bioAY4a and other lignocellulolytic fungi; suggesting AAJ6 is a high CAZyme producer. We expressed the glycoside hydrolase families GH74 and GH3 from plasmid or genome-integrated to evaluate the ethanol production from cellulosic substrates in Brazilian industrial Saccharomyces cerevisiae strains (PE-2 and SA-1) evolved for thermotolerance (AMY12 and AMY35). Those expressing the genome-integrated enzymes showed the highest β-glucosidase activity and growth in medium with cellobiose at 40°C. The strain AGY005 (integrated cassettes) showed 19, 23 and 46% higher ethanol production in SHF, pSSF (partial hydrolysis SSF) and SSF processes, respectively, using Avicel, and ∼50% more ethanol using pre-treated sugarcane bagasse, compared to the strain with a plasmid-based expression. These results indicate the improved performance of thermotolerant industrial strains with genome-integrated CAZymes in the SSF process for 2G ethanol. |
| Databáze: | OpenAIRE |
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