| Autor: |
Kayvan Samimi, Tongcheng Qian, Dan L. Pham, Emmanuel Contreras Guzman, Melissa C. Skala, Steven M. Trier |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Label-free Biomedical Imaging and Sensing (LBIS) 2021. |
| Popis: |
A single photon-excited confocal whole-cell autofluorescence lifetime system is described that uses fast field-programmable gate array (FPGA)-based time tagging electronics to implement time-correlated single photon counting (TCSPC) with simultaneous near-IR brightfield imaging. This system resolves variations in the fluorescence decay of the metabolic coenzyme NAD(P)H that provides high accuracy and precision for classifying activated and quiescent primary human T cells (ROC AUC = 0.98). This performance is similar to that obtained using commercial two-photon fluorescence lifetime imaging microscopy (FLIM). The small footprint, low cost, and high acquisition speed make this system attractive for monitoring cell biomanufacturing. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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