Effects of Hydrogen Ion Dissociation and Concentration on The Reactivity of Dichromate-Fixed Tissue Components to the Pas Procedure: Recommendations for Reducing Undesirable Background Staining

Autor:	L. L. Vacca, Martha Hogan, Janet L. Hobbs
Rok vydání:	1978
Předmět:	chemistry.chemical_compound Distilled water Chemistry Formaldehyde Periodic acid Organic chemistry Schiff test Anatomy Incubation Dissociation (chemistry) Nuclear chemistry Dilution Staining
Zdroj:	Stain Technology. 53:107-112
ISSN:	0038-9153
Popis:	The undesirable PAS reactivity of cytoplasmic aldehydes after dichromate fixation can be suppressed without affecting selective staining by lowering the pH of Lillie's Cold Schiff reagent to 1.5. Alternatively, dilution of pH 2.2 Cold Schiff reagent with distilled water (1:2) is recommended. Hydrogen ion concentration and dissociation effect the rate of color formation in various PAS positive sites differentially with respect to the time of incubation in Schiff reagent. Based on the experiments, aldehydes exposed in different tissue components appear to be chemically distinct and separable depending on the rate of color formation and duration of incubation in Schiff reagent.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::962023a20aa47179801675ce54894556 https://doi.org/10.3109/10520297809111451 Zobrazit plný text záznamu