Metagenomic and metatranscriptomic analysis reveals enrichment for xenobiotic‐degrading bacterial specialists and xenobiotic‐degrading genes in a Canadian Prairie two‐cell biobed system
| Autor: | Christopher K. Yost, Jennifer N. Russell, Denise Nilsson, Jordyn Bergsveinson, Claire N. Freeman, Benjamin J. Perry, Claudia Sheedy, Larry Braul |
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| Rok vydání: | 2021 |
| Předmět: |
2. Zero hunger
Oligotropha 0303 health sciences food.ingredient biology Mesorhizobium Computational biology 010501 environmental sciences biology.organism_classification 01 natural sciences Agricultural and Biological Sciences (miscellaneous) Sphingopyxis 03 medical and health sciences chemistry.chemical_compound Bioremediation food chemistry Microbial population biology Metagenomics Stenotrophomonas Xenobiotic Ecology Evolution Behavior and Systematics 030304 developmental biology 0105 earth and related environmental sciences |
| Zdroj: | Environmental Microbiology Reports. 13:720-727 |
| ISSN: | 1758-2229 |
| DOI: | 10.1111/1758-2229.12990 |
| Popis: | Biobeds are agriculture-based bioremediation tools used to safely contain and microbially degrade on-farm pesticide waste and rinsate, thereby reducing the negative environmental impacts associated with pesticide use. While these engineered ecosystems demonstrate efficient pesticide removal, the microbiomes in these environments remain largely understudied both taxonomically and functionally. This study used metagenomic and metatranscriptomic techniques to characterize the microbial community in a two-cell Canadian biobed system before and after a field season of pesticide application. These culture-independent approaches identified an enrichment of xenobiotic-degrading bacteria, such as Afipia, Sphingopyxis and Pseudomonas, and enrichment and transcription of xenobiotic-degrading genes, such as peroxidases, oxygenases, and hydroxylases, among others; we were able to directly link the transcription of these genes to Pseudomonas, Oligotropha, Mesorhizobium, Rhodopseudomonas, and Stenotrophomonas taxa. |
| Databáze: | OpenAIRE |
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