Comparison of nuclear hydration in boar spermatozoa before and after freeze-thawing: Contrast analysis of cells embedded in bromide-labeled medium
| Autor: | H. Ekwall, J. L. Courtens, L. Plöen |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
endocrine system
Histology BOAR Analytical chemistry Freeze thawing Cryopreservation law.invention 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Bromide law medicine Instrumentation 030304 developmental biology 0303 health sciences 030219 obstetrics & reproductive medicine urogenital system Chemistry Sperm Chromatin Medical Laboratory Technology Cell nucleus medicine.anatomical_structure Biophysics Anatomy Electron microscope |
| Zdroj: | Microscopy Research and Technique. 21:249-254 |
| ISSN: | 1059-910X |
| DOI: | 10.1002/jemt.1070210308 |
| Popis: | Epon labeled with bromide was used to embed ejaculated and freeze-thawed spermatozoa, with the hypothesis that it replaces most of cell water. Image analysis of relative contrasts between sperm nuclei and the surrounding medium revealed that when used in low concentrations, bromide is mostly adsorbed to the nuclear structures. For higher concentrations, the chromatin is saturated, and the increase in contrast can be used to calculate relative differences in the hydration of nuclei. Boar sperm nuclei are more hydrated after freeze-thawing than before. |
| Databáze: | OpenAIRE |
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