In vitro cytotoxicity, cell cycle arrest, and antioxidation studies of ruthenium(II) complex [Ru(dmb)2(AHPIP)](ClO4)2
| Autor: | Hong-Liang Huang, De-Gang Xing, Yun-Jun Liu, Yang-Yin Xie, Gan-Jian Lin, Guang-Bin Jiang, Yan Zhang, Shu-Yong Deng |
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| Rok vydání: | 2014 |
| Předmět: |
Cell cycle checkpoint
biology Chemistry Stereochemistry Electrospray ionization Organic Chemistry Acridine orange Cell cycle biology.organism_classification HeLa chemistry.chemical_compound Apoptosis Hydroxyl radical General Pharmacology Toxicology and Pharmaceutics Ethidium bromide Nuclear chemistry |
| Zdroj: | Medicinal Chemistry Research. 23:4376-4382 |
| ISSN: | 1554-8120 1054-2523 |
| DOI: | 10.1007/s00044-014-1000-6 |
| Popis: | A new Ru(II) complex [Ru(dmb)2(AHPIP)](ClO4)2 (Ru1) was synthesized and characterized by elemental analysis, electrospray ionization mass spectra, and 1H NMR and 13C NMR. The cytotoxicity in vitro toward BEL-7402, HeLa, MCF-7, and MG-63 cells was studied by MTT method. The complex shows moderate cytotoxic activity and the IC50 values are 20.2 (±1.6), 16.8 (±1.3), 39.9 (±2.5), and 46.7 (±2.0) μM toward BEL-7402, HeLa, MCF-7, and MG-63 cell lines, respectively. Apoptosis was studied by acridine orange/ethidium bromide staining and flow cytometry. The cellular uptake showed that the complex can enter into the cytoplasm. The cell cycle arrest showed that Ru1 inhibits the proliferation of BEL-7402 cells in the G0/G1 phase. In addition, the antioxidant activity of the complex against hydroxyl radical (·OH) was also investigated. |
| Databáze: | OpenAIRE |
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