Decrease Tyrosine Phosphorylation of Stat3 in Human Myocardium with End-Stage Congestive Heart Failure

Autor:	J. S. McLaughlin, C. Wei
Rok vydání:	2000
Předmět:	medicine.medical_specialty biology business.industry Tyrosine phosphorylation medicine.disease Human myocardium chemistry.chemical_compound chemistry Internal medicine Heart failure cardiovascular system Cardiology medicine biology.protein Stage (cooking) business STAT3 Instrumentation
Zdroj:	Microscopy and Microanalysis. 6:596-597
ISSN:	1435-8115 1431-9276
DOI:	10.1017/s1431927600035479
Popis:	Recent study demonstrated that decrease signal transducer and activator of transcription-3 (STAT3) phosphorylation and increase apoptosis might be a critical point in the transition between compensatory cardiac hypertrophy and heart failure. To date, the protein expression of STAT3 in normal and failing human heart remains unclear. Therefore, the current study was designed to investigate the protein expression of STAT3 in human myocardium with end-stage congestive heart failure (CHF) and compared with that in normal human cardiac tissue.Human cardiac atrial tissue was obtained from normal subjects (n=5) and end-stage CHF patients (n=5) during cardiac transplantation. To detect the DNA fragmentation, in situ terminal deoxymucleotidyl transferase dUTP nick end labeling (TUNEL) was performed. An average of 1000 nuclei was analyzed for TUNEL study. STAT3 protein expression and phosphorylation of STAT3 were determined by immunohistochemical staining (IHCS) with total STAT3 and phospho-specific STAT3 antibodies.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::95fb1cfc7859c27d1a1c836ab36c2f7c https://doi.org/10.1017/s1431927600035479 Zobrazit plný text záznamu