Autor:	Dominique Lombardo, Jean Hardwigsen, Yves Patrice Le Treut, Benkoel L, Dodero F, Chamlian A, Danielle Botta-Fridlund, P. Campan
Rok vydání:	2001
Předmět:	chemistry.chemical_classification Reactive oxygen species Pathology medicine.medical_specialty Contraction (grammar) Physiology Gastroenterology Ischemia Biology medicine.disease Microfilament digestive system Staining law.invention chemistry Confocal microscopy law medicine Perfusion Actin
Zdroj:	Digestive Diseases and Sciences. 46:1663-1667
ISSN:	0163-2116
DOI:	10.1023/a:1010693218680
Popis:	We studied and quantified the effect of ischemia-reperfusion on hepatic F-actin on bile canalicular and basolateral membranes in human liver allografts by means of confocal laser scanning microscopy imaging. The phalloidin-FITC staining of F-actin was normal in liver hepatocytes before reperfusion but decreased significantly after reperfusion (by 25% of controls). These results indicate that hepatic F-actin alteration is produced during the reperfusion phase. This modification, probably induced by reactive oxygen species, could impair bile canalicular contraction and tight junction permeability and consequently bile secretion in the postoperative period.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::95f4e19e7d07c69a2a894a46c29dab35 https://doi.org/10.1023/a:1010693218680 Zobrazit plný text záznamu Full text from SpringerLink