Molecular Detection and Characterization of p44/msp2 Multigene Family of Anaplasma phagocytophilum from Haemaphysalis longicornis in Mie Prefecture, Japan
| Autor: | Shigehiro Akachi, Shuichi Masuda, Yuko Shimamura, Shigetoshi Sakabe, Eri Onoda, Takashi Kanda, Hongru Su, Norio Ohashi, Saori Oishi, Ayaka Sato, Fuyuki Abe, Hiromi Fujita |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) biology Obligate Phylogenetic tree Human granulocytic anaplasmosis 030106 microbiology General Medicine Amplicon Tick bacterial infections and mycoses biology.organism_classification medicine.disease Anaplasma phagocytophilum Virology 03 medical and health sciences 0302 clinical medicine Infectious Diseases parasitic diseases medicine 030212 general & internal medicine Haemaphysalis longicornis Nymph |
| Zdroj: | Japanese Journal of Infectious Diseases. 72:199-202 |
| ISSN: | 1884-2836 1344-6304 |
| DOI: | 10.7883/yoken.jjid.2018.485 |
| Popis: | Anaplasma phagocytophilum is an obligate intracellular bacterium that causes human granulocytic anaplasmosis (HGA), an emerging tick-borne infectious disease. This bacterium expresses various 44-kDa major outer membrane proteins encoded by the p44/msp2 multigene family to avoid the host immune system. We previously detected A. phagocytophilum p44/msp2 from the tick Haemaphysalis longicornis in Mie Prefecture, Japan in 2008. In this study, we further investigated a total of 483 H. longicornis ticks (220 adults and 263 nymphs) collected from the Mie Prefecture by PCR targeting p44/msp2 to characterize the p44/msp2 multigene family of A. phagocytophilum. Six of the 483 ticks tested were PCR-positive for A. phagocytophilum p44/msp2, and these positive individuals were at the nymph stage of the tick life cycle. Cloning, sequencing, and phylogenetic analyses of the amplicons revealed that the 11 p44/msp2 clones obtained from the positive ticks shared a 54.9%-99.3% amino acid sequence similarity with the 27 previously identified clones from HGA patients in Japan. In particular, 6 p44/msp2 clones displayed the highest similarities (97.2%-99.3%) with 3 previously identified clones (FJ417343, FJ417345, FJ417357). Thus, the data from this study provide important public health information regarding A. phagocytophilum infection transmitted by H. longicornis ticks, especially at the nymph stage. |
| Databáze: | OpenAIRE |
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