The application of hollow fibre-liquid phase micro-extraction on the bioassay experiment of oestrogen chemicals
| Autor: | Myun Joo Lee, Jiho Lee, Hyun Young Kim, Seung H. Yu, Sang Don Kim |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Chromatography Chemistry Health Toxicology and Mutagenesis Extraction (chemistry) Public Health Environmental and Occupational Health Soil Science Salt (chemistry) Pollution Toluene Yeast Analytical Chemistry Solvent chemistry.chemical_compound Phase (matter) Environmental Chemistry Bioassay Waste Management and Disposal Water Science and Technology Dichloromethane |
| Zdroj: | International Journal of Environmental Analytical Chemistry. 92:255-267 |
| ISSN: | 1029-0397 0306-7319 |
| DOI: | 10.1080/03067319.2010.484889 |
| Popis: | The object of this study was to improve pre-concentration techniques using hollow fibre protected-liquid phase micro-extraction (LPME) for its application on biotic samples and limited environmental samples provided in small volumes. Five parameters, including extraction time, type of solvent, extents of agitation, fibre length and salt content of HF-LPME were optimised for the yeast two-hybrid assay and gas chromatography-mass spectrometer (GC/MS) chemical analysis. The results of chemical analysis were compared to the results of a bioassay using the yeast two-hybrid system to analyse endocrine-disrupting chemicals (EDCs). For the yeast two-hybrid assay, the optimised parameters comprised of an extraction time of 40 min, toluene as the extraction solvent, a stirring rate of 500 rpm, a fibre length of 2.0 cm and a salt concentration of 20% (w/v). For GC/MS analysis, HF-LPME conditions were optimised with an extraction time of 40 min, toluene: dichloromethane at a ratio of 1 : 1 (v : v) as the extraction s... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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