| Autor: |
Pei Yang, Yujing Zhai, Jian Ni, Changhai Qu, Jingchen Tian, Jing Fu, Hui Zhang, Sali Cao, Jin Zhang, Juanjuan Qi, Xingbin Yin |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Journal of Medicinal Plants Research. 6 |
| ISSN: |
1996-0875 |
| DOI: |
10.5897/jmpr12.290 |
| Popis: |
For the first time, a rapid and specific reversed phase-high performance liquid chromatography-ultraviolet (RP-HPLC-UV) method was developed for the analysis of polyphyllin I and polyphyllin II in beagle dog plasma. The method was applied to study the pharmacokinetics of Rhizoma paridis capsule containing polyphyllin I and polyphyllin II. The analysis was carried out on a Diamonsil C18 (2) reversed-phase column (250 ´ 4.60 mm, 5 μm) by isocratic elution with acetonitrile and 0.1% phosphoric acid (50: 50, v/v). The flow rate was 1.00 ml/min and the detection wavelength was set at 203 nm. Linear responses were obtained for polyphyllin I ranging from 2 to 300 µg/ml. The intra-and inter-day precisions (RSDs) were less than 9.23 and 9.24% with the accuracy (%) ranging from 87.65 to 107.25%. The extraction recovery ranged from 85.07 to 91.82% with RSD less than 5.55%. Stability studies showed that polyphyllin I and polyphyllin II were stable in preparation and analytical process. The results indicated that the validated method was successfully used to determine the concentration-time profiles of polyphyllin I and polyphyllin II. Key words: Polyphyllin I, polyphyllin II, pharmacokinetics, reversed phase-high performance liquid chromatography (RP-HPLC), beagle dog plasma. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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