Preliminary X-ray diffraction analysis of crystalline lectins from the seeds and leaves of Sophora japonica
| Autor: | C N Hankins, A McPherson, L Shannon |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Sophora
biology Molecular mass Stereochemistry food and beverages Lectin Cell Biology Polyethylene glycol biology.organism_classification Biochemistry Crystal chemistry.chemical_compound chemistry visual_art X-ray crystallography visual_art.visual_art_medium biology.protein Molecule Bark Molecular Biology |
| Zdroj: | Journal of Biological Chemistry. 262:1791-1794 |
| ISSN: | 0021-9258 |
| DOI: | 10.1016/s0021-9258(19)75708-1 |
| Popis: | The Japanese Pogada Tree (Sophora japonica) contains at least four distinct lectins distributed among seeds, bark, and leaf tissues of the plant. All of these lectins are N-acetylgalactosamine-specific, have molecular weights of about 130,000, are glycoproteins and possess substantial sequence homology. However, they are products of distinct genes. We have crystallized Sophora lectins from bark, seeds, and two different lectins from leaves. Multiple crystal forms of each variety have been obtained by vapor diffusion with polyethylene glycol 4,000 solutions, and five of the crystal forms have been characterized by X-ray diffraction. The data demonstrates that at least in the case of leaf lectin II, the tetrameric molecule contains at least one exact dyad axis. Several of the crystals appear suitable for high resolution structure analysis. |
| Databáze: | OpenAIRE |
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