A Single Nucleotide Polymorphism in the MGMT Gene is a Novel Prognostic Biomarker in Patients with Metastatic Colorectal Cancer
| Autor: | Jay Nayak, Arjun Sood, Sanjay Goel, Amartej Merla, Radhashree Maitra, Titto Augustine, Mahadi A. Baig, Santiago Aparo, John M. Mariadason, David M Reynolds |
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| Rok vydání: | 2015 |
| Předmět: |
Sanger sequencing
Oncology medicine.medical_specialty Environmental Engineering Microarray business.industry Colorectal cancer Single-nucleotide polymorphism medicine.disease Industrial and Manufacturing Engineering Metastasis symbols.namesake Internal medicine Genotype Multiplex polymerase chain reaction medicine symbols SNP business |
| Zdroj: | British Journal of Medicine and Medical Research. 5:604-611 |
| ISSN: | 2231-0614 |
| Popis: | Background: Discovery of novel biomarkers of prognosis and drug response remains an elusive, yet critical goal. Thus, accurate and rapid screening of an array of pertinent mutations/SNPs is an essential step in cancer management. Methods: Using a high-throughput multiplex PCR microarray technique, we simultaneously screened the mutational status/SNP of 32 hotspots in multiple genes for metastatic colorectal cancer (mCRC) from 126 formalin fixed paraffin embedded samples from 78 patients. The efficacy of the technology was validated by cross-comparison with conventional Sanger sequencing and Original Research Article |
| Databáze: | OpenAIRE |
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