İnsan İmmün Yetmezlik Virüsü (HIV) Enfeksiyonu Tanısında Enzim ‘Immunoassay’ (EIA), İmmünoblot ve HIV RNA Polimeraz Zincir Reaksiyonu Test Sonuçlarının Değerlendirilmesi
| Autor: | İmran Sağlık, Dilek Colak, Dilara Inan, Gözde Öngüt, Derya Mutlu, Rabia Can Sarınoğlu |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Microbiology (medical)
chemistry.chemical_classification General Immunology and Microbiology biology medicine.diagnostic_test business.industry Human immunodeficiency virus (HIV) virus diseases medicine.disease_cause Roche Diagnostics medicine.disease Virology law.invention Infectious Diseases Enzyme Acquired immunodeficiency syndrome (AIDS) chemistry law Immunoassay medicine Mann–Whitney U test biology.protein Antibody business Polymerase chain reaction |
| Zdroj: | Mikrobiyoloji Bulteni. 53:401-407 |
| ISSN: | 0374-9096 |
| DOI: | 10.5578/mb.68323 |
| Popis: | Acquired Immunodeficiency syndrome (AIDS) is an important global public health issue. Increasing HIV/AIDS cases reported each year has become a serious health problem for our country. The fourth generation enzyme immunoassay (EIA) test is the first step in the laboratory diagnosis of human immunodeficiency virus (HIV) infection. When the EIA test is repeatedly reactive, antibody-based tests such as immuno blot (IB), line immunoassay (LIA), HIV 1-2 antibody differentiation immunoassay, and HIV RNA tests for the early period of infection are used as confirmatory tests. The aim of this study was to evaluate the results of three different methods for the diagnosis of HIV infection. HIV 1-2 IB and quantitative HIV-1 RNA PCR tests were performed in 199 patient samples. These samples were detected as the reactive or gray zone with HIV 1-2 Ab+Ag EIA test between 2010 and 2015 at Akdeniz University Hospital, Microbiology Laboratory. HIV 1-2 Ab+Ag determination in serum samples was performed with the EIA method (Elecsys HIV combi PT test, Roche Diagnostics, Germany). A commercial kit (INNO-LIA HIV I-II Score, Innogenetics, Belgium) was used for HIV 1-2 IB method. The presence of HIV-1 RNA was investigated by automated nucleic acid extraction and real-time PCR method (Ampliprep/COBAS Tagman HIV-1 Test, Roche Diagnostics, Germany) in plasma samples. For statistical analysis, SPSS, Mann Whitney U test was used, ROC analysis was performed and p 10.000.000, > 10.000.000 and 5.040.000 copies/ml, respectively). For the kit that we used for HIV 1-2 Ab+Ag EIA COI ratio of >16.45 had a sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of 97.6%, 98.1%, 97.6% and 98.1%, respectively for the detection of HIV infection (r= 0.994, p 10.000.000, 5.040.000 and > 10.000.000 copies/ml whose EIA tests were repeatedly reactive. HIV IB test was detected as the gray zone in one of them and as negative in the remaining two (HIV EIA S/CO values were 265, 9.5 and 131.8, respectively). These patients were diagnosed as acute HIV infection with clinical and laboratory findings. In conclusion, HIV RNA should also be performed and included in the diagnostic algorithm for acute HIV infection. |
| Databáze: | OpenAIRE |
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