Isolation and Identification of Bacillus amyloliquefaciens IBFCBF-1 with Potential for Biological Control of Phytophthora Blight and Growth Promotion of Pepper
Autor: | Mengjun Zhang, Airong Shen, Xue Zhaodong, Jilie Li, Shiyong Tan, Zhun Yan, Yu Yongting, Taimeng Tan, Zeng Liangbin |
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Rok vydání: | 2016 |
Předmět: |
0106 biological sciences
0301 basic medicine Rhizosphere biology Bacillus amyloliquefaciens Physiology fungi food and beverages Plant Science biology.organism_classification 16S ribosomal RNA 01 natural sciences Microbiology 03 medical and health sciences Horticulture 030104 developmental biology Phytophthora capsici Pepper Genetics Blight Phytophthora Agronomy and Crop Science Bacteria 010606 plant biology & botany |
Zdroj: | Journal of Phytopathology. 164:1012-1021 |
ISSN: | 0931-1785 |
Popis: | In this study, 76 bacterial strains were isolated from the rhizosphere soil of pepper. Of these, 23 bacterial isolates capable of inhibiting Phytophthora capsici growth were selected. Among the antagonistic bacteria, one strain, IBFCBF-1 showed the strongest antagonistic activity, and was identified as Bacillus amyloliquefaciens based on the results of 16S rRNA gene sequence analysis, physiological and biochemical testing, and morphological characteristics. When tested with a dual-culture method and with laboratory greenhouse studies, the strain IBFCBF-1 was found to be a potential biocontrol agent for controlling the plant pathogen, P. capsici. Moreover, it showed high efficiency and broad-spectrum antifungal properties in vitro. Under greenhouse conditions, IBFCBF-1 could significantly promote the growth of pepper seedlings, and was able to solubilize phosphate, and produce indole acetic acid (IAA) and ammonia. This study clearly demonstrated that IBFCBF-1 is a potential candidate exhibiting phytophthora blight-suppressive and plant growth-promoting effects on pepper. |
Databáze: | OpenAIRE |
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