Fad24, a Positive Regulator of Adipogenesis, Is Required for S Phase Re-entry of C2C12 Myoblasts Arrested in G0 Phase and Involved in p27Kip1 Expression at the Protein Level
| Autor: | Shigehiro Osada, Masayoshi Imagawa, Makoto Nishizuka, Natsuki Ochiai |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pharmacology Gene knockdown Pharmaceutical Science Skeletal muscle General Medicine Cell cycle Biology Cell biology 03 medical and health sciences Histone acetyltransferase binding 030104 developmental biology medicine.anatomical_structure Adipogenesis Immunology medicine Myocyte ORC1 C2C12 |
| Zdroj: | Biological & Pharmaceutical Bulletin. 39:807-814 |
| ISSN: | 1347-5215 0918-6158 |
| Popis: | Factor for adipocyte differentiation 24 (fad24) is a positive regulator of adipogenesis. We previously found that human fad24 is abundantly expressed in skeletal muscle. However, the function of fad24 in skeletal muscle remains largely unknown. Because skeletal muscle is a highly regenerative tissue, we focused on the function of fad24 in skeletal muscle regeneration. In this paper, we investigated the role of fad24 in the cell cycle re-entry of quiescent C2C12 myoblasts-mimicked satellite cells. The expression levels of fad24 and histone acetyltransferase binding to ORC1 (hbo1), a FAD24-interacting factor, were elevated at the early phase of the regeneration process in response to cardiotoxin-induced muscle injury. The knockdown of fad24 inhibited the proliferation of quiescent myoblasts, whereas fad24 knockdown did not affect differentiation. S phase entry following serum activation is abrogated by fad24 knockdown in quiescent cells. Furthermore, fad24 knockdown cells show a marked accumulation of p27(Kip1) protein. These results suggest that fad24 may have an important role in the S phase re-entry of quiescent C2C12 cells through the regulation of p27(Kip1) at the protein level. |
| Databáze: | OpenAIRE |
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