Method to extract multiple states in F 1 -ATPase rotation experiments from jump distributions
| Autor: | Kaicheng Zhu, Sándor Volkán-Kacsó, Rudolph A. Marcus, Luan Q. Le, Haibin Su |
|---|---|
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Proceedings of the National Academy of Sciences. 116:25456-25461 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1915314116 |
| Popis: | Significance In single-molecule imaging of the F 1 - ATPase rotation by nanoprobes, there are jumps in the angular position. Their distribution is used in a method for detecting hidden states in the transitions during rotation steps. Complementing stalling experiments, this method reveals, now in free rotation without magnetic tweezers, that the motor can be in either of 2 states at a rotation angle. A comparison between the experimental trajectories and a multistate theory reveals that an 80° substep of the coupled ATP binding and ADP release involves an intermediate state reminiscent of a 3-occupancy structure. Its lifetime ( ∼ 10 μs) is comparable with the frame time of the imaging, so, by detecting this short-lived state, the method provides an increased effective time resolution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|