Whole-genome, transcriptome, and methylome analyses provide insights into the evolution of platycoside biosynthesis in Platycodon grandiflorus, a medicinal plant
Autor: | Byoung-Ohg Ahn, Yurry Um, Tae-Jin Yang, Soo-Jin Kwon, Jong Bhak, Dong Jin Lee, Ok Tae Kim, Dae Young Lee, Chang-Kug Kim, Sang-Ho Kang, Seung-il Yoo, Yi Lee, Keunpyo Lee, Chang Pyo Hong, Jae-Pil Choi, Sin-Gi Park, Oksung Chung, In-gang Shin, Jungho Lee, Geum-Soog Kim, Jungeun Kim |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
Genetics 0303 health sciences Campanulaceae biology Plant Science Platycodon grandiflorus Horticulture biology.organism_classification 01 natural sciences Biochemistry Genome Transcriptome 03 medical and health sciences Gene family Epigenetics Gene Orthologous Gene 030304 developmental biology 010606 plant biology & botany Biotechnology |
Zdroj: | Horticulture Research. 7 |
ISSN: | 2052-7276 2662-6810 |
Popis: | Triterpenoid saponins (TSs) are common plant defense phytochemicals with potential pharmaceutical properties. Platycodon grandiflorus (Campanulaceae) has been traditionally used to treat bronchitis and asthma in East Asia. The oleanane-type TSs, platycosides, are a major component of the P. grandiflorus root extract. Recent studies show that platycosides exhibit anti-inflammatory, antiobesity, anticancer, antiviral, and antiallergy properties. However, the evolutionary history of platycoside biosynthesis genes remains unknown. In this study, we sequenced the genome of P. grandiflorus and investigated the genes involved in platycoside biosynthesis. The draft genome of P. grandiflorus is 680.1 Mb long and contains 40,017 protein-coding genes. Genomic analysis revealed that the CYP716 family genes play a major role in platycoside oxidation. The CYP716 gene family of P. grandiflorus was much larger than that of other Asterid species. Orthologous gene annotation also revealed the expansion of β-amyrin synthases (bASs) in P. grandiflorus, which was confirmed by tissue-specific gene expression. In these expanded gene families, we identified key genes showing preferential expression in roots and association with platycoside biosynthesis. In addition, whole-genome bisulfite sequencing showed that CYP716 and bAS genes are hypomethylated in P. grandiflorus, suggesting that epigenetic modification of these two gene families affects platycoside biosynthesis. Thus whole-genome, transcriptome, and methylome data of P. grandiflorus provide novel insights into the regulation of platycoside biosynthesis by CYP716 and bAS gene families. |
Databáze: | OpenAIRE |
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