308 Screening Strawberry in Vitro for Response to Colletotrichum acutatum

Autor:	Barbara J. Smith, F.A. Hammerschlag, Margery Koch-Dean, Sandra Garces, John Maas
Rok vydání:	2000
Předmět:	Horticulture Colletotrichum acutatum Biology biology.organism_classification In vitro
Zdroj:	HortScience. 35:445B-445
ISSN:	2327-9834 0018-5345
Popis:	As part of a program to generate anthracnose (Colletotrichum acutatum) resistance in strawberry (Fragaria × ananassa Duch.) via either tissue culture or gene transfer techniques, studies were conducted to determine whether in vitro screening for resistance to C. acutatum was feasible. Six commercial cultivars (Latestar, Delmarvel, Pelican, Sweet Charlie, Chandler, and Honeoye) that differed in their response to the pathogen under field conditions were tested to see whether this response was reflected in vitro. Leaves from 4-week-old shoot cultures were soaked in a spore suspension of C. acutatum isolate Goff, transferred to 0.5% water agar, and the presence or absence of disease symptoms was evaluated on a 0-4 rating scale after 7 days. Five of the six cultivars exhibited a disease rating similar to field results. This study suggests that there is potential to use this procedure as a screening technique, and studies are in progress to screen strawberry regenerants for resistance.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::955b6da8a48e1ddc11d715322ac44c9b https://doi.org/10.21273/hortsci.35.3.445b Zobrazit plný text záznamu