Isolation of nuclear, chloroplast and mitochondrial DNA from the moss Physcomitrella patens
| Autor: | Christiane Friese, J.R. Marienfeld, Ralf Reski, Wolfgang O. Abel |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Genetics
Mitochondrial DNA food and beverages Plant Science General Medicine Biology Physcomitrella patens biology.organism_classification Nuclear DNA Chloroplast chemistry.chemical_compound Cell nucleus medicine.anatomical_structure Chloroplast DNA chemistry Biochemistry Organelle medicine Agronomy and Crop Science DNA |
| Zdroj: | Plant Science. 61:235-244 |
| ISSN: | 0168-9452 |
| DOI: | 10.1016/0168-9452(89)90230-6 |
| Popis: | In this report efficient, rapid and easy methods for the isolation of nuclee, chloroplasts and mitochondria and their corresponding DNAs from a large scale culture of the moss Physcomitrella patens are described. Microscopical controls of organelle preparations revealed single and intact nuclei, chloroplasts and mitochondria. Per 10 g fressh weight average yields of 15 μg nuclear chloroplast DNA and 1 μg mitochondrial DNA were obtained. The purity of organelle DNA was tested by hybridization with non-corresponding organelle specific clones. Isolation of nuclei and mitochondria from mosses and characterization of their DNAs are presented for the first time. |
| Databáze: | OpenAIRE |
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