Overexpression of the StP5CS gene promotes nodulation and nitrogen fixation in vegetable soybean under drought stress
| Autor: | Shou-ping Yang, Feng Gao, Yue-Lin Zhu, Xuan Wang, Junyi Gai |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Transgene fungi Wild type food and beverages Soil Science Plant Science 01 natural sciences 03 medical and health sciences Horticulture 030104 developmental biology Glutamine synthetase Glycine Nitrogen fixation Proline Leghemoglobin Agronomy and Crop Science Gene 010606 plant biology & botany |
| Zdroj: | LEGUME RESEARCH - AN INTERNATIONAL JOURNAL. |
| ISSN: | 0976-0571 0250-5371 |
| DOI: | 10.18805/lr-490 |
| Popis: | The StP5CS (GenBank accession number: JN606861) T6 homozygous transgenic lines (HTLs) of vegetable soybean [Glycine max (L.) Merrill] were grown using vermiculite pot culture to determine whether StP5CS overexpression would enhance nodulation and symbiotic nitrogen fixation (SNF) in two T6 HTLs (17W-1, 17W-2) under drought conditions. The growth performance, nodule development and seed weight of T6 HTLs were significantly better than those of wild type (WT) plants. The proline levels in various tissues of T6 HTLs were higher than WT plants. The concentrations of total ureide, total N, leghemoglobin (Lb) and the activity of glutamine synthetase (GS, EC 6.3.1.2) in the T6 HTLs were significantly increased. Moreover, the relative expression levels of five key nodulation- and SNF-associated genes (i.e., GmENOD40-1, GmENOD40-2, GmLba, GmGS1â1 and GmGS1â2) were significantly higher in T6 HTLs. In conclusion, overexpression of StP5CS enhances nodulation and SNF in transgenic vegetable soybean under drought stress conditions |
| Databáze: | OpenAIRE |
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