Clinical analysis by proton spin-echo NMR. 2. Oxidation of intracellular glutathione as a consequence of penicillamine therapy in rheumatoid arthritis
| Autor: | W. E. Smith, John Reglinski, M. Brzeski, M. Marabani, R. D. Sturrock |
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| Rok vydání: | 1992 |
| Předmět: |
Chemotherapy
medicine.medical_specialty Chemistry medicine.medical_treatment Penicillamine Nuclear magnetic resonance spectroscopy Glutathione medicine.disease Red blood cell chemistry.chemical_compound Endocrinology medicine.anatomical_structure Mechanism of action Rheumatoid arthritis Internal medicine Drug Discovery medicine Molecular Medicine medicine.symptom Intracellular medicine.drug |
| Zdroj: | Journal of Medicinal Chemistry. 35:2134-2137 |
| ISSN: | 1520-4804 0022-2623 |
| DOI: | 10.1021/jm00089a026 |
| Popis: | Spin echo NMR analysis is used to monitor the effect of penicillamine on intact erythrocytes obtained from patients suffering from rheumatoid arthritis during a 12-week period of therapy. The results are compared to the previously reported in vitro effects of the compound (McKay, C. N. N.; et al. Biochim. Biophys. Acta 1986, 888, 30-35). At clinical assessment at week 12, the 20 patients were divided into responder and nonresponder groups. The intracellular glutathione in the responder group is more oxidized (P less than 0.01) than in the nonresponder group. A retrospective analysis of the two patient groups at the initial assessment following the commencement of therapy indicated that in the nonresponder group intracellular glutathione was significantly more reduced (P less than 0.02) than in the responder group. It is postulated that penicillamine stimulates cellular defense against the oxidation of the cell membrane at the expense of cytosolic glutathione. This initial study suggests that spin-echo NMR analysis of erythrocyte glutathione can act as an early indicator of a clinical response to penicillamine therapy. |
| Databáze: | OpenAIRE |
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