Abstract P4-06-09: Genetic Signature Discriminating Metastatic from Non-Metastatic Small Tumors
| Autor: | Cd. Shriver, Brad Love, RE Ellsworth, Allyson L. Valente, LA Field, Jennifer Kane, Lorraine Tafra |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Cancer Research. 70:P4-06 |
| ISSN: | 1538-7445 0008-5472 |
| Popis: | Background: The widespread use of mammographic screening resulted in increased diagnosis of small ( Methods: Frozen breast specimens were collected from women with T1 tumors and either negative (n=29) or positive (n=15) lymph node status. RNA was isolated from pure tumor cell populations after laser microdissection. Gene expression data was generated using HG U133A 2.0 arrays (Affymetrix). Differential expression was determined using Mann-Whitney testing using a P-value < 0.001 to define significance. Results for ESR1 were validated by immunohistochemistry. Results: Tumor characteristics did not differ significantly between groups in terms of age at diagnosis, grade, HER2 or PR status; however, tumors from patients with positive lymph nodes (47%) were significantly (P4X higher levels in tumors without metastasis, in agreement with IHC findings. Conclusions: Small metastatic tumors differ in gene expression from those without metastasis. EPHX2 has been implicated as a metastasis suppressor while AURKA has been implicated as a metastasis promoter. These results suggest that small tumors have different propensities to metastasize and the genetic signature may serve as a new molecular tool to discriminate metastatic and non-metastatic small tumors, allowing appropriate treatment and risk assessment to be performed. Citation Information: Cancer Res 2010;70(24 Suppl):Abstract nr P4-06-09. |
| Databáze: | OpenAIRE |
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